Sort Sprites with a Custom Sorting Axis

To sort and render tile spritesA 2D graphic objects. If you are used to working in 3D, Sprites are essentially just standard textures but there are special techniques for combining and managing sprite textures for efficiency and convenience during development. More info
See in Glossary on an Isometric Z as Y TilemapA GameObject that allows you to quickly create 2D levels using tiles and a grid overlay. More info
See in Glossary, set the Transparency Sort Axis to a Custom Axis. To do this:

1. Set the Tilemap Renderer component Mode to Individual.
2. Navigate to Edit > Project Settings > Graphics.
3. Set Transparency Sort Mode to Custom Axis.
4. Set the Transparency Sort Axis Y-value to 1 and the Z-value to –0.26.

Refer to the page on Creating an Isometric Tilemap for more information about the Transparency Sort Axis settings.